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. 2021 Mar 9;30(6):485–499. doi: 10.1093/hmg/ddab064

Figure 1.

Figure 1

Knockdown of SOX9 results in an increase of ciliary gene expression and a decrease in expression of genes negatively regulating proliferation. (A) Western blot and (B) immunofluorescence confirms knockdown of SOX9 protein following SOX9 siRNA treatment. (C) A volcano plot of adjusted P-value versus fold change upon SOX9 knockdown indicates that 93 genes exhibit significantly altered expression (33 decreased and 60 increased). (D) Quantitative PCR confirms all but one (SKIV2L) of the top five upregulated and top five downregulated genes observed with RNA-seq. Error bars are standard deviation from three biological replicates. (E) GO analysis of downregulated genes reveals a role for SOX9 in Notch signaling as well as in the regulation of proliferation. (F) GO analysis of upregulated genes is enriched for ciliary development and function. Neg, Negative; Pos, Positive; reg, regulation. Scale bar size is 100 μM (B).