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. 2021 Mar 9;30(6):485–499. doi: 10.1093/hmg/ddab064

Figure 4.

Figure 4

EPCAM is direct target of SOX9. (A) RNA-seq (blue) and ChIP-seq (red) reads over the EPCAM locus confirm siRNA-mediated EPCAM knockdown and SOX9 binding at the transcription start site. (B, C) Immunofluorescence detection of SOX9 and EPCAM in PANC-1 s treated with control siRNA (B) and SOX9 siRNA (C). (D) Positive correlation of EPCAM and SOX9 expression in TCGA pancreatic adenocarcinoma samples. (E-G) Whole mount in situ hybridization to detect epcam expression in the pancreas of 5 dpf zebrafish with the following sox9b genotypes: (E) +/+, (F) +/− and (G) −/− (H–M) cilia phenotype in MZ epcamjh79 mutants: Bright-field images of otic vesicles in wild-type (H) and MZ epcam mutants (K). Immunofluorescent detection of acetylated tubulin (green), phalloidin (red) and DAPI (blue), using confocal microscopy, in wild-type (I) and MZepcam mutants (L). Dashed insets indicate areas enlarged in (J) and (M) to visualize cilia. Scale bar 100 μm (B, C, E, F, G, H, I, K and L) and 5 μm (J, M).