Table 6.

Pup body weight development and attainment of puberty

Dietary dose (mg/kg bw per day)	F1				F2
	Control	100	300	1,000	Control	100	300	1,000
Birth weight males (g)	7.19a	7.26a	7.25a	7.22a	7.01	6.94	6.59	6.87
PND 4	9.96a	9.86a	9.83a	9.77a	9.68	9.58	8.94	9.34
PND 14	32.34a	30.76a	30.61a	31.41a	–	–	–	–
PND 21	53.49a	51.92a	49.56a	51.02a	–	–	–	–
AGD/body weight cubed PND 4	17.0a	16.5a	17.1a	17.5a	15.92	15.38	15.87	15.53
Nipple retention PND 13b	0	2	3	0	–	–	–	–
Age (PND) at balanopreputial gland cleavage C1Ac	22.3	22.6	22.1	22.2	–	–	–	–
Body weight (g) on day of balanopreputial gland cleavage C1A	59.1	60.4	55.4	57.3
Age (PND) at balanopreputial gland cleavage C1Bc	22.3	22.4	22.2	22.4
Body weight (g) on day of balanopreputial gland cleavage C1B	59.2	58.4	56.4	58.4
Birth weight females (g)a	6.80a	6.88a	6.96a	6.82a	6.55	6.55	6.22	6.59
PND 4	9.30a	9.41a	9.62a	9.34a	9.17	9.15	8.66	9.02
PND 14	30.99a	29.43a	29.87a	29.98a	–	–	–	–
PND 21	50.67a	49.52a	48.48a	49.18a	–	–	–	–
AGD/body weight PND 4	8.2a	8.4a	8.5a	8.5a	7.70	7.64	7.52	7.70
Age (PND) at vaginal opening C1A	33.6	32.8	32.8	32.8	–	–	–	–
Body weight (g) on day of vaginal opening C1A	123.0	117.8	112.4d	117.5
Age (PND) at vaginal opening C1B	33.2	32.7	32.9	32.6
Body weight (g) on day of vaginal opening C1B	120.6	120.2	114.2	117.1

bw: body weight; PND: postnatal day; AGD: anogenital distance.

^aCalculated by the Panel for the pups of F0 dams based on individual data.

^bCalculated by the Panel based on individual data; Litters with > 0 male pups with more than 2 retained nipples.

^cThe Panel noted that this test as conducted by the laboratory does not comply with the OECD TG 443 and cannot be considered a measure of puberty in male.

^dp ≤ 0.05 (ANOVA and Dunnett).