Figure 2.

RNA sequencing analysis of flow cytometry sorted cells shows temporal dynamics in CD11b⁺Ly6C^hi monocytes function following SP-C^I73T induced injury. RNA-sequencing of cell sorted CD11b⁺Ly6C^hi monocytes from control (CTL, oil treated SP-C^I73T mice) or SP-C^I73T mice 3 d and 14 d after injury. Total RNA was prepared and analyzed by RNA-seq. N = 4 was utilized in each study condition. (A), Two-dimension principle component analysis (PCA) of gene expression variance among monocytes accumulating in the lung in control (CTL) or SP-C^I73T mice 3 d and 14 d after injury. (B) Positively enriched pathways at 3 d post injury (normalized enrichment score, NES); (C) Negatively enriched pathways at 14 d post injury.