TABLE 1:
NES consensus sequences.
| Class | Consensusa | Number of residues between Φs | Select cargoes that bind CRM1 using this consensusb (NES ID in NESdb) |
|---|---|---|---|
| 1a | Φ1XXXΦ2XXΦ3XΦ4 | 3-2-1 | PKI (5), MEK1 (11), HDAC5c (61), Paxillin (136), 4E-T (233) |
| 1b | Φ1XXΦ2XXΦ3XΦ4 | 2-2-1 | |
| 1c | Φ1XXXΦ2XXXΦ3XΦ4 | 3-3-1 | SNUPN (1) |
| 1d | Φ1XXΦ2XXXΦ3XΦ4 | 2-3-1 | |
| 2 | Φ1XΦ2XXΦ3XΦ4 | 1-2-1 | HIV-Rev (2), SMAD4 (18), FMRP (22) |
| 3 | Φ1XXΦ2XXXΦ3XXΦ4 | 2-3-2 | mDia2 (77), CDC7 (197) |
| 4 | Φ1XXXΦ2XXΦ3XXXΦ4 | 3-2-3 | X11L2 (141) |
| 1a-R | Φ1XΦ2XXΦ3XXXΦ4 | 1-2-3 | hRio2 (153), CPEB4 (198) |
| 1b-R | Φ1XΦ2XXΦ3XXΦ4 | 1-2-2 | |
| 1c-R | Φ1XΦ2XXXΦ3XXXΦ4 | 1-3-3 | |
| 1d-R | Φ1XΦ2XXXΦ3XXΦ4 | 1-3-2 |
aΦ is Leu, Val, Ile, Phe, or Met and X is any amino acid.
bSome NES sequences match multiple consensus patterns. CRM1-bound crystal structures of the NESs noted here reveal the correct consensus classes (Dong et al., 2009; Monecke et al., 2009; Guttler et al., 2010; Koyama et al., 2014; Fung et al., 2015, 2017; Baumhardt et al., 2020).
cUses a Thr instead of Val as Φ1 (Fung et al., 2017).