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. Author manuscript; available in PMC: 2021 May 6.
Published in final edited form as: Nat Chem Biol. 2020 Dec 16;17(1):10–19. doi: 10.1038/s41589-020-00700-7

Figure 6. Engineered regulation of CRISPR-Cas biogenesis and interference.

Figure 6.

Customized transcriptional (a), post-transcriptional (a and c), and post-translational (b and d) control strategies enable precise spatiotemporal regulation of CRISPR-Cas activity. Engineering CRISPR-Cas elements to automatically and predictably sense and respond to a wide range of extra- and intracellular molecules could usher in a new era of biology, where these genetically-encoded tools illuminate the relationship between the current state of a cell and its past molecular history. a, describes how molecular signals can control expression/biogenesis of crispr components b-d, examples of how CRISPR-Cas systems have been engineered to achieve tight control of the CRISPR interference pathway. Created with BioRender.com