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Lamellipodia dynamics are slowed in fibroblasts expressing constitutively active Rac1 and RhoA. Time lapse sequence of transiently transfected primary fibroblasts expressing either GFP-tagged constitutively active Rac1 (GFP-Rac1-CA), GFP-Rac1-CA and mScarlet-tagged constitutively active RhoA (mScar-RhoA-CA). Quantified in Figure 4. Images were captured at one frame/4 s using a spinning-disk confocal microscope. The video plays at 21 frames/s.
