FIGURE 3:

Both Asi1 and Hrd1 ubiquitinate Sec61-2-GFP in vivo. The indicated strains expressing Sec61-2-GFP were grown into log phase and treated with MG132 or a vehicle control (DMSO). Cells were lysed, and microsomes were collected and immunoprecipitated with α-GFP. Samples were then subjected to SDS–PAGE and immunoblot by α-ubiquitin and α-GFP. One of three biological replicates is shown.