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. 2021 May 1;32(10):1048–1063. doi: 10.1091/mbc.E21-02-0068

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FIGURE 4: — Wild-type cells do not commit to mutants with constitutively mobile polarity sites. (A, B) Selected time points from movies of mating mixes imaged at 30°C. Cartoons indicate cells in the selected montages at the start of the displayed imaging interval. Yellow oval: mother–bud neck. White arrowhead: weak mobile Bem1-tdTomato clusters in wild-type partners. Orange arrowheads: stably oriented Bem1 clusters characteristic of committed cells. Dashed outline: fused zygote. (A) MATα wild-type cells (DLY12944; magenta) mixed with MATa wild-type cells (DLY9069; green). (B) MATα wild-type cells (DLY12944; magenta) mixed with MATa mutants that form constitutively mobile polarity clusters (cdc24-m1 rsr1Δ, DLY22797; green). (C, D) Spatial autocorrelation traces of representative wild-type (C) or cdc24-m1 rsr1Δ (D) cells mixed with wild-type α partners. Traces represent cells with the underlined genotype (a). (E, F) Spatial autocorrelation traces of representative wild-type α cells mixed with wild-type (E) or cdc24-m1 rsr1Δ (F) partners. Horizontal yellow line: threshold autocorrelation used to call commitment. Purple vertical line: commitment time as scored visually. Wild-type cells attempting to mate with constitutively indecisive mutants did not reach the threshold.