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. 2021 May 1;32(10):1048–1063. doi: 10.1091/mbc.E21-02-0068

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© 2021 Clark-Cotton et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 6: — Wild-type cells do not commit to unpolarized partners. Selected time points from movies of mating mixes. B, bud; yellow oval, mother–bud neck. White arrowhead: weak, mobile clusters in wild-type partners. Orange arrowheads: stably oriented Bem1 clusters characteristic of committed cells. Dashed outline: fused zygote. 10 μM α-factor was added to B and C to sustain G1 arrest of mutant MATa cells. (A) MATα wild-type cells (DLY9070; green) mixed with MATa wild-type cells (DLY12943; magenta), imaged at 37°C. (B) The same MATα wild-type strain mixed with cdc24-4^ts ste20^ΔCRIB MATa cells (DLY23256; nuclear accumulation of Whi5-GFP, green, indicates G1 cells), imaged at 35°C. (C) MATα wild-type cells (DLY12944; magenta) mixed with MATa cells harboring membrane-targeted, constitutively active Cdc24 (MT-GFP-CDC24^38A, DLY23351) that do not make polarity clusters. Scale bars: 5 µm. (D) Example spatial autocorrelation traces from wild-type cells mixed with the indicated partners. Horizontal yellow line: threshold autocorrelation used to call commitment. Purple vertical line: commitment time as scored visually. Wild-type cells attempting to mate with unpolarized mutants did not reach the threshold, even after 100 min.