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. 2021 Apr 22;11:643104. doi: 10.3389/fonc.2021.643104

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Copyright © 2021 Jiang, Liu, Xu, Liang, Yu, Liao, Chen, Huang, Sun, Yi, Zhang, Lu, Wang, Chen, Chen, Li, Yao, Chen, Guo, Liu and Zhan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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qRT-PCR and immunohistology. (A–C) plots show the expression of these three genes in two types of osteosarcoma cells as well as in normal control cells, respectively. (D1–J2) plots demonstrate the expression of these three genes in osteosarcoma and paraneoplastic tissues (guide inverted microscopy 100×). The (K–M) plots represent the statistics of the positivity rate of these three genes in pathological sections, respectively. (J) has been used to number the immunohistochemically stained images. “*” represents P < 0.05, “**” represents P < 0.01, “***” represents P < 0.001.