FIGURE 4.

BAK1 is a functional target of miR‐125b in the response of APL cells to chemotherapy. (A) qRT‐PCR analysis of ROS production‐related enzyme genes in NB4‐LV‐miR‐NC and NB4‐LV‐miR‐125b cells. (B) Changes of BMF, BAK1, and BBC3 expression in NB4 cells upon miR‐125b overexpression by qRT‐PCR. (C) qRT‐PCR analysis of BAK1 in NB4‐LV‐miR‐NC and NB4‐LV‐miR‐125b cells treated with 2 μM ATO for 48 h. Knockdown efficiency (D), apoptosis rate (E), and ROS levels (F) in NB4 cells were analyzed after knocking down the BAK1 gene followed by ATO (2 μM) treatment. (G) Proposed model depicting regulation and role of NRF2/miR‐125b‐1 in APL