Figure 3.

TCDD-exposed female mice lack an appropriate compensatory hyperinsulinemic response to HFD feeding but show normal insulin sensitivity. Glucose-stimulated plasma insulin levels were assessed in vivo at week 8, and insulin tolerance at week 5 and 9 (see Fig. 1A for study timeline). Plasma insulin levels during a GTT at week 8 of the study in (A) females and (B) males (n = 5-8 per group). Blood glucose levels during an ITT at (C, D) 5 weeks and (E, F) 9 weeks in (C, E) females and (D, F) males (n = 7-8 per group). Data are presented as mean ± SEM in line graphs or median with min/max values in box and whisker plots. Individual data points on box and whisker plots represent biological replicates (different mice). *P < 0.05, **P < 0.01, colored stars are versus COChow. The following statistical tests were used: (A-F) line graphs, two-way RM ANOVA with Tukey’s multiple comparison test; box and whisker plots, two-way ANOVA with Tukey’s multiple comparison test.