Figure 4.

TCDD exposure has sex-specific effects on islet histology in mice. Whole pancreas was harvested at week 12 for analysis by immunofluorescent staining (see Fig. 1A for study timeline). (A, D) Average islet area, (B, E) % insulin⁺ area / islet area, (C, F) % glucagon⁺ area / islet area in (A-C) females and (D-F) males (n = 4-5 per group). (G) Representative images of pancreas sections showing immunofluorescence staining for insulin and glucagon. Scale bar = 100 µm. All data are presented as box and whisker plots with median and min/max values. Individual data points on box and whisker plots represent biological replicates (different mice). The following statistical test was used: two-way ANOVA with Tukey’s multiple comparison test.