Fig 2. Neuronal expression of the wild-type VPS24 protein rescues the vps24¹ TS paralytic phenotype as well as locomotor and lifespan deficits.

(A) The TS paralytic phenotype of the vps24 mutant was rescued by expression of the wild-type EGFP-VPS24 in neurons but not in muscle or glia. The GAL4 drivers for neuronal, muscle and glial expression were Appl-GAL4, Mhc-GAL4 and Repo-Gal4 respectively. (B) Climbing tests, carried out at a room temperature (RT) of 22–24°C, indicated no detectable climbing ability in the vps24 mutant. The climbing tests were truncated at 2 min if 50% climbing had not occurred and zero was given for the climbing index. (C) Loss of the VPS24 function reduced lifespan with respect to WT when raised at a permissive temperature of 20°C. These mutant phenotypes were rescued by expression of wild-type EGFP-VPS24 in neurons but not in muscle or glia. The same GAL4 drivers were used for subsequent cell-type specific expression studies.