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. 2021 May 6;17(5):e1009557. doi: 10.1371/journal.ppat.1009557

Fig 5. Neutrophil recruitment is increased in RTX-treated corneas.

Fig 5

A. Flow cytometry analysis of cellular infiltrates in infected corneas from RTX-treated and vehicle-treated mice. Panels describe the gating strategy to identify myeloid subpopulations. PMNs were defined on FSC vs SSC gate, as CD45+, live cells that express CD11b and Ly6G. B. Quantification of the absolute cell counts reflecting populations of total CD45+ infiltrates (first panel), Ly6G+CD11b- (second panel) and Ly6G+CD11b+ (third panel, neutrophils). Bar graphs show mean values; symbols represent individual mouse. Data are from N = 6 infected corneas (1 per mouse) per condition representative of two independent experiments. P-values by Student’s t-test. Cumulatively, data show increased cellularity in the infected RTX-treated mice.