Fig 6. ZAP proteins from tetrapods have antiviral activity in the presence of cognate TRIM25.

(A) Phylogenetic analysis of TRIM25 protein sequences from vertebrate species. Shaded areas indicate cluster sequences from mammals (in blue), birds and reptiles (in green) and ray-finned fish (in yellow). (B) HEK293T ZAP^-/- and TRIM25^-/- cells were co-transfected with an HIV-1_CG proviral plasmid as well as a fixed amount of a plasmid encoding human ZAP or ZAP chimeras from various vertebrate species. Animal names indicate source of the RNA-binding domains for each ZAP chimera. For each ZAP protein, cells were also co-transfected increasing amounts of a plasmid (0ng 10ng, 30ng or 90ng) expressing a TRIM25 protein from the 6 different indicated species. After 48h, infectious virus yield was determined using MT4-R5-GFP target cells.