Fig 4. Intra-community spread is common for GA1 and GA2 loci.

A. Number of communities per dataset, classified by GA1 and GA2 loci presence/absence. Numbers in parentheses under each dataset name indicate total number of volunteers analyzed in that collection. *Includes CL08 and CL14 which were screened by PCR as described in the text. B. Percentage of communities in the CGR and BIOML collections that show the indicated event, out of the total communities in that dataset that have at least one strain with the indicated GA type: no transfer, single-species spread (present in two species), multi-species spread (present in three species or more). C. Communities with a single-species intra-community transfer (highlighted in light grey) or multi-species spread (highlighted in dark grey). Letters in parentheses indicate type of GA2. Numbers listed separately in an individual box indicate the T6SS regions are different (<99.99% DNA sequence identity and/or divergent variable regions). **Screened by PCR and sequencing of variable region, as described in the text. Species determined by 16S rRNA gene sequence. ‡The two relevant strains from donor AF15 and AF16 are nearly clonal and appear to have both a GA2b and GA2c (see details in text).