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. 2021 Apr 26;17(4):e1009327. doi: 10.1371/journal.pgen.1009327

Fig 3. AURKA is specifically required in oocytes to complete meiosis I.

Fig 3

(A) Representative confocal images of oocytes and eggs retrieved from oviducts of WT and Aurka KO females or oocytes matured in vitro. Cells were immunostained with antibodies against α-Tubulin (green) and DAPI (gray) (B) Quantification of percentage (%) of cells ovulated at Metaphase II (Met II); (Unpaired Students t-Test, two-tailed, **** p<0.0001). (C) Quantification of the % of oocytes that undergo polar body extrusion (PBE) in vitro (Unpaired Students t-Test, two-tailed, **** p<0.0001). (D) Quantification of the % of oocytes that undergo nuclear envelope breakdown (NEBD) in vitro (Unpaired Students t-Test, two-tailed, p = 0.6707). Graphs show the mean ± SEM from 3 independent experiments (3 females/genotype). Scale bars: 10μm. (E) Live cell light-sheet imaging of WT and KO oocytes expressing histone H2B-mCHERRY (magenta) and stained with SiR-tubulin (green). Some KO oocytes also expressed exogenous AURKA-EGFP, AURKB-EGFP or AURKC-EYFP (gray). Maximum intensity z-projections and selected time points are shown. White arrow indicates AURKC-EYFP at aMTOC. Scale bars: 10 μm. (F) Data in (E) was used to quantify % of oocytes arrested at Metaphase I. Graph shows the mean ± 95% confidence interval. n (WT, KO, KO + Aurka, KO + Aurkb, KO + Aurkc) = 10, 10, 13, 15, 11, respectively.