Fig 6. Inserting a flanking hsp70 core promoter induces non-coding transcription that is not processive, and causes little repression of the eve promoter.

eve-lacZ and flanking hsp-driven RNA expression in stage 5 embryos from eve pseudo-loci flanked by an NEE-hsp70-GFP cassette, inserted at the same MiMIC site as in Fig 4, and in the same orientation. The 3 loci (map above each one) differ only in the presence (A, NEE-hspGFPwt) or absence (B, NEE-hspGFPΔ and C, NEE-hspGFPinvΔ) of homie, and in the orientation of the NEE-hspGFP cassette (pointing to the left in A and B, and to the right in C). Positions and orientations of the RNA probes used are shown as black and red arrows in the map in A (and in B and C for the GFP probe). Embryos were stained with the probes listed in A, using either black lettering corresponding to black arrows in the map (detecting transcription from right to left), or red lettering corresponding to red arrows (detecting transcription from left to right).