Figure 3.

Estimating the core TX–TL parameters. Experimental data is from [25,47]. Shaded regions indicate standard error over three replicates (left), and model simulations based on the inferred parameters (right). (A) Decay of purified deGFP-MGapt transcripts initiated at six different mRNA concentrations. (B) Transcription kinetics reported by a Malachite Green aptamer. (C) Translation kinetics reported by deGFP. Rows (B) and (C) show four different concentration of plasmid DNA that was added to each TX–TL master mix at the beginning of the experiment.