KEY RESOURCES TABLE
REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Bacterial and virus strains | ||
AAV2/5-EF1a-DIO-hChR2(H134R)-EYFP | University of North Carolina Vector Core | N/A |
AAV-EF1a-DIO hChR2 (E123T/T159C)-p2A-EYFP-WPRE | University of North Carolina Vector Core | N/A |
Chemicals, peptides, and recombinant proteins | ||
Chicken anti-GFP Antibody | Abcam | RRID:AB_300798 |
Rabbit anti-Fos Antibody | Cell Signaling Technology | RRID:AB_2247211 |
Rabbit GABA Polyclonal Antibody | Invitrogen | RRID:AB_2549714 |
Rabbit PV Antibody | Invitrogen | RRID:AB_2173898 |
Rabbit SSt Antibody | Invitrogen | RRID:AB_2633039 |
Rabbit GAD Antibody | Cell Signaling Technology | RRID:AB_10835855 |
Goat Alexa 488 anti-Chicken Antibody | Abcam | RRID:AB_2636803 |
Alexa-555 anti-Rabbit Antibody | Invitrogen | RRID:AB_2536100 |
NeuroTrace 640 | Invitrogen | RRID:AB_2572212 |
Sheep Anti-Digoxigenin Antibody | Millipore Sigma | RRID:AB_2734716 |
Anti-GFP Antibody | Abcam | RRID:AB_303395 |
Fast Red TR/Naphthol AS/MX | Sigma-Aldrich | F4648 |
Donkey Alexa 488 anti-Rabbit Secondary Antibody | Jackson ImmunoResearch Laboratories | RRID:AB_2313584 |
Vectashield | Vector Laboratories | N/A |
Fluoromount-G | Thermo-Fisher | 00-4958-02 |
TTX | Tocris | 1078 |
NBQX | Tocris | 0373 |
D-APV | Tocris | 0106 |
Gabazine | Tocris | 1262 |
TPMPA | Tocris | 1040 |
Experimental models: Organisms/strains | ||
Emx1-Cre Mice | The Jackson Laboratory | RRID:IMSR_JAX:005628 |
Software and algorithms | ||
Spike2 | Cambridge Electronic Design | N/A |
MATLAB | MathWorks | N/A |
Igor Pro | WaveMetrics | N/A |
AxoGraphX | AxoGraph | N/A |
ImageJ | Fiji | N/A |
Other | ||
Optical Fiber Patch Cable | Thorlabs | MR81L01 |
Optical Fiber for Optrode | Thorlabs | FT200EMT |
Ceramic Ferrule for Optrode | Precision Fiber Products | MM-FER2002S15 |
Silver Wire for Optrode | A-M Systems | 786500 |
Connector Pins for Optrode | A-M Systems | 520200 |
473 nm Laser | Shanghai Laser & Optics Century | N/A |
Laser Power Meter | Thorlabs | PM100D |
Laser Power Sensor | Thorlabs | S121C |
Metabond | Parkell | S398, S371 and S399 |
AC/DC Differential Amplifier | A-M Systems | Model 3000 |
Data Acquisition System | Cambridge Electronic Design | Power1401–3A |