Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Anti-CDP/Cux1 (rabbit polyclonal) | Santa Cruz | Cat# SC-13024, RRID:AB_2261231 |
IF (1:200) |
| Antibody | Anti-Foxp2 (rabbit polyclonal) | Abcam | Cat# ab16046, RRID:AB_2107107 | IF (1:500) |
| Antibody | Anti-Tbr1 (rabbit polyclonal) | Abcam | Cat# ab31940, RRID:AB_2200219 | IF (1:500) |
| Antibody | Anti-Tbr2 (rabbit polyclonal) | Abcam | Cat# ab23345, RRID:AB_778267 | IF (1:500) |
| Antibody | Anti-Pax6 (rabbit polyclonal) | Covance | Cat# PRB-278P, RRID:AB_291612 |
IF (1:500) |
| Antibody | Anti-γ-tubulin (rabbit polyclonal) | Abcam | Cat# ab11317, RRID:AB_297921 | IF (1:500) |
| Antibody | Anti-Diaph3 (rabbit polyclonal) | Tominaga et al., 2000 | IF (1:500) WB (1:500) |
|
| Antibody | Anti-Spag5 (rabbit polyclonal) | Proteintech | Cat# 14726–1-AP, RRID:AB_2194787 | IF (1:200) |
| Antibody | Anti-α-tubulin (mouse monoclonal) | Sigma | Cat# T6199, RRID:AB_477583 | IF (1:500) |
| Antibody | Anti-cleaved caspase-3 (Alexa Fluor 488conjugate) (rabbit monoclonal) | Cell Signaling | Cat# 9603, RRID:AB_11179205 | IF (1:100) |
| Antibody | Anti-Ki67 (mouse monoclonal) | BD phamingen | Cat# 556003, RRID:AB_396287 | IF (1:500) |
| Antibody | Anti-SPAG5 (rabbit polyclonal) | Sigma | Cat# HPA022008, RRID:AB_1853519 | WB (1:750) |
| Antibody | Anti-GAPDH (chicken polyclonal) | Millipore | Cat# AB2302, RRID:AB_10615768 | WB (1:2000) |
| Antibody | Anti-NUMA (mouse monoclonal) | BD Biosciences | Cat# 610561, RRID:AB_397913 | WB (1:500) |
| Antibody | Anti-PAR3 (rabbit polyclonal) | Millipore | Cat# 07–330, RRID:AB_2101325 | IF (1:500) WB (1:500) |
| Antibody | Anti-GPSM2 (rabbit polyclonal) | Ezan et al., 2013 | IF (1 :200) WB (1 :200) |
|
| Antibody | Anti-CENPA (rabbit monoclonal) | Cell Signaling | Cat# 2048, RRID:AB_1147629 | WB (1 :500) |
| Antibody | Anti-Dynein IC1/2 (mouse monoclonal) | Santa Cruz | Cat# sc-13524, RRID:AB_668849 | WB (1:500) |
| Antibody | Anti-KNSTRN (rabbit polyclonal) | Sigma | Cat# HPA042027, RRID:AB_10797378 | IF (1:1000) WB (1:1000) |
| Antibody | Anti-INSC (rabbit polyclonal) | Abcam | Cat# ab102953, RRID:AB_10711784 | WB (1:1000) |
| Antibody | Anti-NUMB (goat polyclonal) | Abcam | Cat# ab4147, RRID:AB_304320 | IF (1:100) WB (1:100) |
| Antibody | Anti-p150Glued /dynactin (mouse polyclonal) |
BD Biosciences | Cat# 610474, RRID:AB_397846 | WB (1:500) |
| Antibody | Anti-CLASP1 (rabbit monoclonal) | Abcam | Cat# ab108620, RRID:AB_10864427 | WB (1:5000) |
| Antibody | Anti-BrdU (rat monoclonal) | Serotec | Cat# MCA2060GA, RRID:AB_10545551 | IF (1:200) |
| Antibody | Anti-Ki67 (rabbit polyclonal) | Abcam | Cat# ab15580, RRID:AB_443209 | IF (1:250) |
| Antibody | Anti-Tbr1 (chicken polyclonal) | Millipore | Cat# AB2261, RRID:AB_10615497 | IF (1:100) |
| Antibody | Anti-SPAG5 (rabbit polyclonal) | NovusBio | Cat# NB100-74638, RRID:AB_2239831 |
WB (1:1000) |
| Antibody | Alexa Fluor 488 anti-mouse IgG (goat polyclonal) | Invitrogen | Cat# A11017, RRID:AB_143160 | IF (1:800) |
| Antibody | Alexa Fluor 488 anti-rabbit IgG (goat polyclonal) | Invitrogen | Cat# A11034, RRID:AB_2576217 | IF (1:1000) |
| Antibody | Alexa Fluor 488 anti-chicken IgY (IgG) (goat polyclonal) | Jackson ImmunoResearch | Cat# 103-545-155, RRID:AB_2337390 | IF (1:1000) |
| Antibody | Alexa Fluor 568 anti-mouse IgG (goat polyclonal) | Invitrogen | Cat# A21124, RRID:AB_141611 | IF (1:1000) |
| Antibody | Alexa Fluor 568 anti-rabbit IgG (goat polyclonal) | Invitrogen | Cat# A11036, RRID:AB_10563566 | IF (1:1000) |
| Antibody | Anti-chicken IgY/HRP-linked (rabbit polyclonal) | Upstate Biotech | Cat# 12–341, RRID:AB_390189 | WB (1:1000) |
| Antibody | Anti-mouse IgG/HRP-linked (goat polyclonal) | Dako | Cat# P0447, RRID:AB_2617137 | WB (1:1000) |
| Antibody | Anti-rabbit IgG/HRP-linked (goat polyclonal) | Cell Signaling | Cat# 7074, RRID:AB_2099233 | WB (1:1000) |
| Cell line (Homo-sapiens) | U2OS cell (osteosarcoma) | ATCC | Cat# HTB-96, RRID:CVCL_0042 | |
| Cell line (Mus musculus) | NIH3T3 immortalized MEFs |
ATCC | Cat# CRL-1658 RRID:CVCL_KS54 |
|
| Strain, strain background | Mus musculus | Jackson Lab | Emx1tm1(cre)Krj/J RRID:IMSR_JAX:005628 | |
| Strain, strain background | Mus musculus | This paper | Diaph3Emx1-Cre cKO (Diaph3f/f; Emx1-Cre) | |
| Transfected construct (human) | Scrambled shRNA | Origene | Cat# TR30012 | Transfected construct |
| Transfected construct (human) | sh-Diaph3 | Origene | Cat# TR304992 | Transfected construct |
| Transfected construct (human) | sh-Spag5 | Origene | Cat# TR309161 | Transfected construct |
| Transfected construct (Mus musculus) |
sh-Spag5 | Origene | Cat# TR509034 | Transfected construct |
| Commercial assay or kit | Mycoplasma PCR detection kit | Sigma | Cat#, MP0035 | |
| Commercial assay or kit | RNeasy Kit | Qiagen | Cat# 74004 | |
| Commercial assay or kit | RT cDNA synthesis Kit | Promega | Cat# A5003 | |
| Commercial assay or kit | SYBR Green SuperMix | Biorad | Cat# 170–8882 | |
| Commercial assay or kit | Lipofectamine LTX | Thermofisher Scientific | Cat# 15338030 | |
| Commercial assay or kit | RNAscope Probe-Mm-Spag5 | ACD | Cat# 505691 | |
| Commercial assay or kit | Pierce BCA protein assay kit | Thermofisher Scientific | Cat# 23225 | |
| Commercial assay or kit | Bolt 4–12% Bis-Tris Plus Gels | Thermofisher Scientific | Cat# NW04125 BOX | |
| Commercial assay or kit | StartingBlock T20 (TBS) blocking buffer | Thermofisher Scientific | Cat# 37543 | |
| Commercial assay or kit | 20x Bolt MOPS SDS running buffer | Thermofisher Scientific | Cat# B0001 | |
| Software, algorithm | Prism | GraphPad, USA | RRID:SCR_002798 | |
| Software, algorithm | Zen lite | Zeiss | ||
| Software, algorithm | Ethovision 6.1, Noldus | Wageningen, The Netherlands |