Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | Clock | NCBI Gene Database |
NCBI Gene: 12753 |
|
| Cell line (Mus musculus) | 4T1 | ATCC | Cat #: CRL-2539; RRID:CVCL_0125 |
|
| Cell line (Homo sapiens) | Lenti-X 293T | Clonetech | Cat #: 632180 | |
| Transfected construct (Mus musculus) | miRDIAN Hairpin Inhibitor | Horizon Discovery | Cat #: IH-310436–08 | |
| Antibody | Rabbit polyclonal anti-CLOCK | Abcam | Cat #: ab3517 RRID:AB_303866 |
WB (1:4000) |
| Antibody | Rabbit polyclonal anti-p84 (THOC1) | Proteintech | Cat #: 10920–1-AP RRID:AB_2202239 |
WB (1:1500) |
| Antibody | Goat polyclonal anti-E-Cadherin | R and D systems | Cat #: AF748 RRID:AB_355568 |
WB (1:1000) |
| Antibody | Rabbit polyclonal anti-Claudin 1 | Proteintech | Cat #: 13050–1-AP RRID:AB_2079881 |
WB (1:2000) |
| Antibody | Mouse monoclonal anti-Vimentin | R and D systems | Cat #: MAB21052 RRID:AB_2832972 |
WB (1:1000) |
| Antibody | Goat polyclonal anti-Actin-HRP | Santa Cruz Biotechnology | Cat #: sc-1616 | WB (1:5000) |
| Antibody | Mouse monoclonal anti-Ki-67 | Agilent Technology | Cat #: M7240 RRID:AB_2142367 |
IHC (1:200) |
| Recombinant DNA reagent | pcDNA3.1(+) (plasmid) | Invitrogen | Cat #: V79020 | |
| Recombinant DNA reagent | pGL4.18 (plasmid) | Promega | Cat #: E6731 | |
| Recombinant DNA reagent | pGL4.13 (plasmid) | Promega | Cat #: E6681 | |
| Recombinant DNA reagent | pRL-SV40 (plasmid) | Promega | Cat #: E2231 | |
| Sequence-based reagent | Primer for sgRNA synthesis | This paper | CCTCTAATA CGACTCACT ATAGGCAAT GGTAGAACT CACACGTTT AAGAGCTAT GC |
|
| Sequence-based reagent | Mouse Clock promoter F | This paper | PCR primers for construction of reporter vector | ATACTCGAGA GGTCACTTG GGTCGT |
| Sequence-based reagent | Mouse Clock promoter R | This paper | PCR primers for construction of reporter vector | ATAAGATCT CCTTCCCCT CCTCCACG |
| Peptide, recombinant protein | Recombinant Cas9 | Clontech | Cat #: Z2641N | |
| Peptide, recombinant protein | Recombinant Mouse TGF-β1 | R and D systems | Cat #: 7666 MB | |
| Commercial assay or kit | ALDEFLUOR Kit | StemCell Technologies | Cat #: ST-01700 | |
| Commercial assay or kit | Lipofectamine LTX and Plus Regent | ThermoFisher SCIENTIFIC | Cat #: 15338100 | |
| Commercial assay or kit | Dual-Luciferase reporter assay system | Promega | Cat #: E1910 | |
| Commercial assay or kit | ReverTra Ace qPCR RT Kit | Toyobo | Cat #: FSQ-201 | |
| Commercial assay or kit | THUNDERBIRD SYBR qPCR Mix | Toyobo | Cat #: QPS-201 | |
| Commercial assay or kit | Taqman MicroRNA Reverse Transcription Kit | Applied Biosystems | Cat #: 4366596 | |
| Commercial assay or kit | Taqman MicroRNA Assay | Applied Biosystems | Cat #: 4427975 | |
| Commercial assay or kit | Guide-it sgRNA in vitro transcription kit | Clontech | Cat #: Z2635N | |
| Commercial assay or kit | Lentiviral High Titer Packaging Mix with pLVSIN series | Takara bio | Cat #: 6952 | |
| Commercial assay or kit | CellTiter-Glo Luminescent cell viability assay | Promega | Cat #: 7572 | |
| Chemical compound, drug | 6-Thioguanine | Fujifilm | Cat #: 203–03771 | |
| Software, algorithm | BD FACS Diva | BD Biosciences | RRID:SCR_001456 | |
| Software, algorithm | FlowJo | BD Biosciences | RRID:SCR_008520 | |
| Software, algorithm | Lumicycle analysis software | Actimetrics | ||
| Software, algorithm | ImageQuant LAS 3000 | GE Healthcare | RRID:SCR_014246 | |
| Software, algorithm | BZ analyzer software | KEYENCE | RRID:SCR_017205 | |
| Software, algorithm | Off-Spotter | Thomas Jefferson University | RRID:SCR_015739 | |
| Software, algorithm | JMP | Statistical Discovery | RRID:SCR_014242 | |
| Other | Hoechst33342 | Dojindo laboratories | Cat #: 346–07951 |