Table 5.
Metabolomic analysis of CFCS.
| Retention time (min) | Substance | MRS area (%) | CFCS area (%) | CFCS area (%) | CFCS area (%) | CFCS area (%) | CFCS area (%) |
|---|---|---|---|---|---|---|---|
| Lc24 | Lf38 | Lf47 | Lp81 | Lp90 | |||
| 4.164 | Carbodiimide | — | 1.48 | 0.74 | 0.89 | 1.51 | 1.37 |
| 4.700 | N.N-Dimethylglycine | 0.04 | — | 0.03 | — | 0.08 | — |
| 7.295 | Lactic acid | 0.85 | 61.77 | 49.18 | 56.43 | 57.64 | 61.05 |
| 7.524 | Acetic acid | — | — | 0.05 | 0.07 | — | 0.04 |
| 7.531 | 2-Hydroxyetanoic acid | — | — | — | — | 0.04 | — |
| 7.768 | Valine | 0.05 | — | — | — | — | — |
| 8.405 | Alanine | 1.14 | 2.15 | 4.09 | 3.40 | 2.24 | 2.00 |
| 9.020 | Glycine | 0.33 | 0.81 | 1.11 | 0.82 | 0.99 | 0.77 |
| 9.873 | β-Lactate | — | — | 0.08 | 0.10 | 0.06 | 0.07 |
| 10.168 | Leucine | 0.17 | — | — | — | — | — |
| 10.445 | 3-Hydroxybutyric acid | — | — | — | — | 0.04 | — |
| 10.638 | α-Hydroxyvaleric acid | — | 0.17 | — | — | 0.10 | 0.16 |
| 12.465 | Valine | 1.02 | 1.71 | 2.85 | 2.95 | 2.12 | 2.06 |
| 13.203 | 4-Methyl-2-hydroxypentanoic acid | — | 0.35 | 0.87 | 0.71 | 0.29 | 0.58 |
| 13.395 | 3-Methyl-2-hydroxypentanoic acid | — | — | — | — | 0.07 | 0.10 |
| 14.510 | Leucine | 2.48 | 2.55 | 3.45 | 4.49 | 3.50 | 3.43 |
| 14.733 | Glycerol | — | 0.71 | — | 1.34 | — | — |
| 14.769 | Phosphoric acid | 7.29 | 7.56 | 11.13 | 9.73 | 7.85 | 7.86 |
| 15.236 | Isoleucine | 1.18 | 1.89 | 2.65 | 2.74 | 2.17 | 2.15 |
| 15.308 | γ-Amino butyric acid | — | — | 0.10 | — | 0.14 | — |
| 15.839 | Butanoic acid | 0.20 | 0.28 | 0.97 | 1.00 | 0.46 | 0.50 |
| 16.220 | 2-Methyl-2.3-dihydroxypropanoic acid | 0.10 | 0.13 | 0.27 | 0.36 | 0.15 | 0.16 |
| 16.705 | Pyrimidine | — | — | 0.13 | — | — | — |
| 17.644 | Serine | 0.75 | 0.85 | 2.23 | 1.19 | 0.61 | 0.47 |
| 18.148 | Butanoic acid | 0.02 | 0.12 | 0.09 | 0.11 | 0.06 | 0.07 |
| 18.272 | 3-Methyl-1.4-dihydroxypiperazine-2.5-dione | — | 0.79 | 0.08 | — | 0.07 | — |
| 18.496 | Butyric acid | — | — | 2.07 | — | — | — |
| 18.531 | Threonine | 0.62 | — | — | 1.71 | 1.38 | 1.24 |
| 18.555 | Lactic acid dimer | — | 0.52 | — | — | 0.25 | 0.27 |
| 19.332 | 2.4-Dihydroxybutanoic acid | — | — | 0.12 | 0.15 | 0.07 | 0.07 |
| 19.494 | Aspartic acid | — | 1.04 | — | 0.64 | — | 0.64 |
| 21.091 | Trisiloxane | — | 0.11 | 0.15 | 0.15 | 0.11 | 0.13 |
| 21.769 | Malic acid | — | 0.12 | — | — | 0.10 | 0.24 |
| 21.865 | 2-Pyrrolidone-5-carboxylic acid | — | 0.34 | — | 0.58 | — | 0.81 |
| 22.262 | Glutamic acid | — | — | — | 0.15 | — | — |
| 22.458 | Methionine | 0.22 | — | 0.27 | — | 0.31 | — |
| 22.588 | Proline | 1.40 | 1.17 | 3.55 | 0.84 | 2.20 | 1.52 |
| 22.708 | Aspartic acid | 0.56 | — | 0.63 | — | 0.90 | 0.20 |
| 23.099 | Phenylalanine | — | 0.92 | — | 1.29 | — | 0.59 |
| 24.376 | Benzenepropanoic acid | — | 0.09 | 0.57 | 0.45 | 0.28 | 0.50 |
| 25.371 | Ornithine | — | — | — | — | 0.21 | — |
| 25.583 | Glutamine | 1.76 | — | 3.81 | — | 3.06 | 1.40 |
| 26.434 | Tartaric acid | 0.34 | 0.40 | 0.67 | 0.47 | 0.49 | 0.54 |
| 26.995 | Hydroxy 4-oxo-2.4-di(hydroxylamine)butanoate | — | — | — | — | 0.16 | — |
| 27.048 | Asparagine | 0.04 | — | 0.36 | — | — | — |
| 27.895 | Lysine | — | — | 1.22 | — | 1.11 | — |
| 28.638 | Arabinitol | — | — | 0.10 | — | — | — |
| 28.805 | Ribitol | — | — | — | — | 0.11 | 0.12 |
| 29.803 | 2.3-Dihydroxypropylphosphoric acid | 0.13 | 0.11 | 0.30 | 0.26 | 0.26 | 0.25 |
| 29.920 | D-Ribo-Hexonic acid | — | — | — | — | 0.09 | 0.11 |
| 30.946 | 2-Keto-D-gluconic acid | 0.7 | — | — | — | 0.06 | — |
| 31.133 | 1,2,3-Propanetricarboxylic acid | — | 6.15 | 0.11 | — | — | — |
| 31.259 | Citric acid | 7.49 | — | — | — | 6.66 | 5.91 |
| 32.345 | Pentanedioic acid | — | 0.34 | 0.75 | 0.16 | 0.29 | — |
| 32.451 | Benzenepropanoic acid | 0.10 | — | — | — | — | — |
| 32.879 | 4-Hydroxyphenyllactic acid | — | — | 0.07 | 0.10 | 0.16 | |
| 33.891 | Tyrosine | 0.13 | — | — | — | — | 0.40 |
| 33.955 | Glucitol | 0.04 | 0.16 | 3.65 | 2.76 | — | |
| 35.660 | Inositol | 31.07 | — | 0.07 | — | — | 0.07 |
| 39.845 | Tryptophan | 0.06 | — | 0.26 | — | 0.16 | — |
| — | Sugars | 38.12 | 3.38 | 4.07 | 2.96 | 0.83 | 1.54 |
| — | Unidentified compounds | 1.60 | 1.81 | 1.20 | 3.83 | 0.62 | 0.46 |
| — | Identified compounds (except sugars) | 60.28 | 94.81 | 94.73 | 93.21 | 98.55 | 98.00 |
| — | Total | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 |