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Effect of incubation period on acid protease production by the isolate Z1LB1.
| No. | Incubation period (in hour) | PA (U/mL) mean ± SEM |
|---|---|---|
| 1 | 24 | — |
| 2 | 48 | 36.8 ± 5a |
| 3 | 72 | 56.3 ± 7b |
| 4 | 96 | 88.0 ± 5.2c |
| 5 | 120 | 95.2 ± 7c |
| 6 | 144 | 71.8 ± 4.9bc |
PA: protease activity; SEM: standard error of the mean. Mean is the average of three measurements. Different letters (a, b, c) designate significantly different means as determined by the SPSS test (P < 0.001); temp. 30°C; initial media pH 4.5.
