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. 2021 May 7;105(10):4153–4165. doi: 10.1007/s00253-021-11281-3

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© The Author(s) 2021

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Fig. 5 — Impurity evaluation of the purified RBD219-N1C1 proteins from three processes. Unpurified (FS) and purified RBD219-N1C1 in reduced SDS-PAGE with Coomassie blue stain (a) and with Western blot using anti-P. pastoris HCP antibody (b). Measured P. pastoris HCP content by quantitative ELISA (c) and endotoxin levels (d) are shown