Table 1.
Summary of the development fermentation runs
| Run # | Fermentation conditions | Endpoint analysis | |||||
|---|---|---|---|---|---|---|---|
| Volume (L) | Medium | Glycerol fed-batch | Total induction time | Biomass (g/L) | OD600 (AU) | Peak yield (mg/L of FS) | |
| 1 | 1 | BSM | No | 70 ± 1 | 417 ± 3 | 260 | 52 ± 2 |
| 2 | 1 | LSM | No | 70 ± 1 | 437 ± 4 | 257 | 237 ± 7 |
| 3 | 1 | LSM | 6 h | 70 ± 1 | 434 ± 3 | 254 | 533 ± 3 |
| 4 | 5 | LSM | 6 h | 87 ± 1 | 413 ± 2 | 230 | 449 ± 8 |
| 5–8* | 5 | LSM | No | 70 ± 2 | 394 ± 20 | 232 ± 11 | 428 ± 36 |
FS fermentation supernatant
*Four reproducibility runs (runs 5–8) were performed. The averages of biomass and yields from four runs are shown