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. Author manuscript; available in PMC: 2021 May 6.
Published in final edited form as: Nat Protoc. 2012 Jan 12;7(2):207–220. doi: 10.1038/nprot.2011.433

Figure 5.

Figure 5.

Characterization of the completed illumination system. Adapted from ref 12. (a) Relative intensity as a function of color pixel value (0–255) for each RBG color plane. (b) Ideal (59.6 um) and measured (68.5 um) width of a defined projection pattern using a 4× objective. This demonstrates spatial spread in illumination due to contrast transfer function of optical components. Width was measured at the point where intensity drops to 10% of the maximum value. (c) Measured spot-size using a 4× objective. This demonstrates a resolution limit of ~14 μm at 4×. (d) Measured spot-size using a 25× objective. This demonstrates a resolution limit of ~5 μm at 25×.