Figure 9.
Serpin E1 deregulation recapitulates the extensive necrosis associated with L30P/R34P overexpression. (A) Serpin E1 levels in the indicated tumors. (B) Serpin E1 levels in plasma and cyst fluid from the indicated cohorts. Plasma and cyst fluids were diluted proportionately, and 40 μg of each was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis. To account for differences in the types of samples present, Ponceau acid red staining of the membrane was used to confirm protein concentrations. (C) Gross appearance of Δ(90)+YAPS127A+serpin E1 tumor sections showing extensive necrosis (arrows) relative to that of a typical Δ(90)+YAPS127A control. (D) Histologic appearance of typical section from the tumor shown in (C) with large pericystic necrotic areas indicated by arrows. (E) Immunoblots for serpin E1 expression in indicated tumor types. Note slower mobility of exogenously expressed, epitope-tagged serpin E1.