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. 2021 Mar 30;35(5):1365–1379. doi: 10.1038/s41375-021-01231-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A Two-dimensional UMAP projection of the transcriptomes of CD45+ lymphocyte cells pooled from three time points from peripheral blood. A total of 24,000 cells are annotated in 21 distinct clusters, 7 of which can be annotated as CD8+ T cells. *STAT3 mutated T-cell cluster. B Dot plot showing the canonical markers used to annotate the clusters. C Volcano plot showing differentially expressed genes between the TCRBV05-01 associated cluster (to the right, significant shown in red) against other CD8+ clusters (to the left, significant shown in blue). D Violin plot showing the scaled STAT3 expression in CD8+ clusters at diagnosis. E Violin plot showing the score of genes having at least one occurence of the transcription factor binding site within 4 kb of STAT3 binding site at diagnosis. F The relative abundances of CD8+ clusters during treatment. G Violin plot showing the significantly downregulated cytotoxic genes (PRF1, GZMB, GZMH, GNLY, LYZ) and stable STAT3 expression in the TCRBV05-01 associated cluster.