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. 2021 Apr 12;40(18):3318–3330. doi: 10.1038/s41388-021-01765-x

Fig. 4. PRDM4 inhibited cell proliferation by regulating cell cycle-related proteins.

Fig. 4

A The differential mRNA expression in HeLa-PRDM4 and HeLa-GFP cells determined by RNA-seq showed 1664 upregulated genes and 510 downregulated genes. B Cell cycle-related gene levels between the HeLa-PRDM4 and HeLa-GFP cells. C The transcript levels of p53, p21, p27, Cyclin D1, and CDK4 were detected in the PRDM4-overexpressing and PRDM4-silenced cells by real-time PCR. D–G Cell cycle proteins were detected by Western blotting, analyzed by utilizing a protein imprinting imaging system (Tanon 5200, China) and expressed as relative expression after normalization to GAPDH. *p < 0.05 and **p < 0.01, while ns indicates no significant difference.