Table 2.
Use of the pBBR1MCS-based plasmid family in Gluconobacter. The studies are grouped by the pBBR1MCS derivative and ordered by the year of publication. cyt. cytochrome; DH dehydrogenase; DHA dihydroxyacetone; GFP green fluorescent protein; P promoter; PPP pentose phosphate pathway; PQQ pyrroloquinoline quinone; SNP single nucleotide polymorphism; SP signal peptide
| Expressed genes | Encoded activity/function | P a | Context | Reference |
|---|---|---|---|---|
| pBBR1MCS-5 | ||||
| tldD, pqqA, pqqABCDE from G. oxydans 621H | PQQ biosynthesis-related enzymes | PtufB, | analysis of PQQ biosynthesis genes in G. oxydans | Hölscher and Görisch 2006 |
| PpqqA, | ||||
| PpqqBCDE | ||||
| gdh, ga5dh from G. oxydans 621H | membrane-bound PQQ-dependent glucose DH and gluconate-5-DH | Plac | increase of gluconate and 5-keto-d-gluconic acid production by G. oxydans strains | Merfort et al. 2006b |
| ga5dh from G. oxydans 621H | membrane-bound PQQ-dependent gluconate-5-DH | PtufB, | increase of 5-keto-d-gluconic acid production by G. oxydans mutant strain | Merfort et al. 2006a |
| Pgdh | ||||
| sldAB from G. oxydans 621H | membrane-bound PQQ-dependent polyol DH | PtufB, | increase of DHA production from glycerol by G. oxydans mutant | Gätgens et al. 2007 |
| Pgdh | ||||
| sldAB from G. oxydans M5 | membrane-bound PQQ-dependent d-sorbitol DH | Plac | characterization of the enzyme in G. oxydans M5 and role in miglitol precursor production | Yang et al. 2008 |
| sldBA from G. suboxydans IFO 3255 | membrane-bound PQQ-dependent d-sorbitol DH | Plac, | characterization of two membrane-bound d-sorbitol DHs and complementation in G. frateurii | Soemphol et al. 2008 |
| PsldBA | ||||
| sldA’B’ from G. oxydans 621H | N-terminal fragments of membrane-bound PQQ-dependent polyol DH | PtufB | construction of the pEXGOX family; a cloning and expression vector system with PtufB from G. oxydans | Schleyer et al. 2008 |
| adhAB from G. oxydans DSM 2003 | membrane-bound PQQ-dependent alcohol DH (mADH) | Plac | enzyme characterization; mADH mutant complementation; lactic acid production from 1,2-propanediol | Wei et al. 2010 |
| gdh from G. oxydans M5 | membrane-bound PQQ-dependent glycerol DH | Plac | enhanced production of DHA by G. oxydans mADH mutant | Li et al. 2010b |
| vgb from Vitreoscilla | Vitreoscilla hemoglobin | PtufB | improvement of cell growth and DHA production by G. oxydans M5 | Li et al. 2010a |
| adhAB from G. oxydans DSM 2003 | membrane-bound PQQ-dependent alcohol DH (mADH) | Padh | characterization of mADH and complementation in G. oxydans DSM 2003 mADH mutant | Wei et al. 2012 |
| xdh from G. oxydans NH-10; gdh from B. subtilis | xylitol DH (XDH) and glucose DH (GDH) | PtufB | improvement of xylitol production by construction of a xdh and gdh co-expressing G. oxydans strain | Zhang et al. 2013 |
| ndh and ga2dh from G. oxydans DSM 2003; gfp | membrane-bound type II NADH DH; FAD-dependent gluconate-2-DH; GFP | PgHp0169, | evaluation of promoter gHp0169 and improvement of 2-keto-d-gluconic acid production by G. oxydans DSM 2003 | Shi et al. 2014 |
| PtufB | ||||
| ga2dh from G. oxydans DSM 2003 | FAD-dependent gluconate-2-DH | PtufB | rational mutagenesis to increase the pBBR1MCS-5 copy number; 2-keto-d-gluconic acid production | Shi et al. 2015 |
| sldAB from G. oxydans 621H; gfp | membrane-bound PQQ-dependent polyol DH; green fluorescent protein | PGOX0169, PGOX0264, PGOX0452, PtufB | promoter strength analysis and 5-keto-d-gluconate accumulation using combinatorial metabolic engineering strategies in industrial strain G. oxydans ZJU2 | Yuan et al. 2016a |
| ga2dh from G. oxydans DSM 2003 | FAD-dependent gluconate-2-DH | PtufB, Pga2dh, PgHp0169 | improvement of 2-keto-d-gluconic acid production by G. oxydans DSM 2003 | Li et al. 2016a |
| adhS and adhABS from G. oxydans DSM 2003 | membrane-bound PQQ-dependent alcohol DH (mADH) | PadhS, PadhAB | study of the AdhS subunit and effect on mADH activity in G. oxydans DSM 2003; hydroxy acid production | Zhang et al. 2016 |
| gcd from X. campestris DSM 3586 | alcohol DH | PGOX0169 | improvement of 5-keto-d-gluconate production by G. oxydans ZJU2 mutant strain | Yuan et al. 2016b |
| zwf, gnd from G. oxydans NH-10 | glucose-6-phosphate DH and 6-phospho-gluconate DH | PtufB | improvement of xylitol production by G. oxydans NH-10; coenzyme regeneration efficiency of the PPP | Li et al. 2016b |
| fusion of L-RI gene from Acinetobacter sp. DL-28; sdh from G. oxydans DSM 2003; gfp | l-ribose isomerase (L-RI) C-terminally fused to PDZ ligand; membrane-bound PQQ-dependent d-sorbitol DH (SDH) N-terminally fused to PDZ domain; GFP fusion protein | PtufB | protein localization; novel strategy for the production of l-erythrose by localizing the assembly of L-RI to SDH via protein-peptide interaction of PDZ ligand and PDZ domain | Zou et al. 2017 |
| adh, idh, aldh, gidh, gdh, pqq3, pqq4, sldAB from G. oxydans 621H | membrane-bound DHs (mDHs) | PGOX1067-68, PGOX1857, | test of promoter strength and enzymatic characterization of mDHs in G. oxydans multi-deletion strain BP.9 | Mientus et al. 2017 |
| genes of putative membrane-bound DHs from mother of vinegar DNA | glucose DH like DH; alcohol DH like DH; polyol DH like DH; aldehyde DH like DH; d-lactate DH like DH | Padh (PGOX1067-68) | heterologous expression of metagenomic mDHs in G. oxydans BP.9 and identification of new substrate specificities | Peters et al. 2017 |
| adhA, adhAB, aldh from G. oxydans DSM 2003 | membrane-bound alcohol DH (mADH) and aldehyde DH (mALDH) | PtufB | characterization of mADH and mALDH; 3-hydroxypropionic acid production | Zhu et al. 2018 |
| sldAB from G. oxydans ZJB-605 | membrane-bound PQQ-dependent d-sorbitol DH | PgHp0169 | improvement of miglitol precursor production by industrial strain G. oxydans ZJB-605 | Ke et al. 2019 |
| trx from G. oxydans NL71 | thioredoxin | Plac | enhancement of G. oxydans resistance to lignocellulosic-derived inhibitors; xylonic acid production | Shen et al. 2020 |
| araC, araE from E. coli MC4100 | transcriptional regulator AraC; l-arabinose-transporter AraE | ParaBAD | plasmid-based tunable l-arabinose-inducible expression system for G. oxydans | Fricke et al. 2020 |
| pqqABCDE and tldD from G. oxydans H-8 | PQQ biosynthesis-related enzymes | PgHp0169 | increase PQQ biosynthesis in repeated batch biotransformation for miglitol precursor production by mutant strain G. oxydans H-8 | Liu et al. 2020a |
| pBBR1MCS-2 | ||||
| gdh from G. oxydans 621H | membrane-bound PQQ-dependent glucose DH | Plac | gluconate and 5-keto-d-gluconic acid accumulation by G. oxydans | Merfort et al. 2006b |
| GOX1857 from G. oxydans 621H | membrane-bound PQQ-dependent inositol DH | PGOX1857 | substrate and co-factor analysis of GOX1857 in G. oxydans 621H | Hölscher et al. 2007 |
| uidA from E. coli DH5α | β-d-glucuronidase UidA | Plac, PGOX0254, PGOX0452 | characterization of constitutive G. oxydans promoter strength | Kallnik et al. 2010 |
| sldA from G. oxydans DSM 7145 | membrane-bound PQQ-dependent polyol / glycerol DH | PsldB | characterization of sldA disruptant mutant and substrate analysis of the enzyme | Voss et al. 2010 |
| mgdh from G. oxydans 621H | membrane-bound PQQ-dependent glucose DH | Pmgdh | evolution for enhanced growth and biotransformations by G. oxydans at low glucose concentration | Zhu et al. 2011 |
| edd-eda and gnd from G. oxydans 621H | 6-phosphogluconate dehydratase; 2-keto-3-deoxy-6-phospho-gluconate aldolase; 6-phosphogluconate DH | PGOX0384 | analysis of cytoplasmic fructose catabolism in a Δgnd mutant lacking the oxidative PPP and Δedd Δeda mutant lacking the Entner-Doudoroff pathway | Richhardt et al. 2012 |
| cyoBACD, cydAB and cydABCD of G. oxydans 621H | quinol terminal oxidases cytochrome bo3 and cytochrome bd | PGOX0384 | influence of terminal oxidases on growth and yield of G. oxydans 621H | Richhardt et al. 2013 |
| GOX0265 of G. oxydans 621H | membrane-bound PQQ-dependent glucose DH (mGHD) with C-terminal Strep-tag | PGOX0452 | gluconate production; influence of mGDH on O2 consumption rate; membrane protein purification by Strep-tactin affinity chromatography | Meyer et al. 2013 |
| phoA and treA from E. coli DH5α | alkaline phosphatase and trehalase fused to selected SPs | PGOX0264 | SP screening in G. oxydans and broadening the substrate range of G. oxydans for growth on trehalose | Kosciow et al. 2014 |
| sldhAB from G. oxydans WSH-003 | membrane-bound PQQ-dependent sorbitol DH (SLDH) | PtufB | enhancing production of l-sorbose from d-sorbitol; increase sldhAB mRNA level by artificial poly-A/T tail | Xu et al. 2014 |
| ndh from G. oxydans DSM 3504 | type II NADH DH (NDH-2) | Pndh | consequence of an additional NADH DH paralog on growth of G. oxydans 621H | Kostner et al. 2015 |
| sdhCDAB, sdhE A. pasteurianus; sdhE from Serratia sp. | succinate DH (SDH) and assembly factor SdhE for attachment of FAD to SdhA |
PGOX0384, PsdhE-Ace, PsdhE-Ser |
SdhE-dependent formation of a functional A. pasteurianus SDH in G. oxydans 621H | Kiefler et al. 2015 |
| GOX1801 from G. oxydans 621H | succinic semialdehyde reductase (SSR) fused C-terminally to Strep-tag | PGOX0264 | characterization of GOX1801 enzyme activities; the first SSR from an aerobic bacterium with high catalytic efficiency | Meyer et al. 2015 |
| sldh from G. oxydans WSH-003; egfp | d-sorbitol DH; enhanced GFP (eGFP), | PB932_2000, PtufB | identification of strong promoters; comparison of PB932_2000 with PtufB form E. coli and from G. oxydans; enhancing production of l-sorbose | Hu et al. 2015 |
| GOX1432 from G. oxydans 621H | cytoplasmic NADP+-dependent mannitol DH fused C-terminally to Strep-tag II | PGOX0264 | role of mannitol DHs in osmoprotection of G. oxydans 621H | Zahid and Deppenmeier 2016 |
| xynA from B. subtilis | endo-1,4-β-xylanase fused N-terminally to the PelB SP | PGOX0264 | xylan utilization; degradation of renewable organic materials and incomplete oxidation for production of value-added products | Kosciow et al. 2016 |
| pqqA, pqqB, pqqC, pqqD, pqqE, tldD from G. oxydans WSH-003 | PQQ biosynthesis proteins | PpqqA, PtufB | analysis of PQQ levels in G. oxydans WSH-003 and related conversion of d-sorbitol to l-sorbose | Wang et al. 2016 |
| sdhCDAB and sdhE from A. pasteurianus; sucCD from Ga. diazotrophicus | succinate DH (SDH) and assembly factor SdhE for attachment of FAD to SdhA; succinyl-CoA synthetase |
PGOX0264, PsdhE |
used for fragment construction for chromosomal insertion of genes to obtain G. oxydans 621H with completed TCA cycle for increased biomass yield from glucose | Kiefler et al. 2017 |
| fdhSCL from G. japonicus; | membrane-bound fructose DH; extracellular sucrase SacC fused N-terminally to the PelB SP | PGOX0264 | production of 5-ketofructose from fructose or sucrose using genetically modified G. oxydans strains | Siemen et al. 2018 |
| sacC from Z. mobilis ZM4 | ||||
| fdhSCL from G. japonicus NBRC3260 | membrane-bound fructose DH (Fdh) | PGOX0264 | production of 5-ketofructose; online monitoring of the respiration activity; pulsed and continuous fructose feed | Herweg et al. 2018 |
| phoA from E. coli; oprF from P. aeruginosa PAO1 | alkaline phosphatase PhoA; first 188 aa of outer membrane porin OprF fused C-terminally to Strep-tag; OprF-PhoA fusion protein | PGOX0264, PGOX0452 | surface display of PhoA in G. oxydans using OprF for delivery and test of linker regions in OprF-PhoA fusion proteins | Blank and Schweiger 2018 |
| codon-optimized sldh from G. oxydans G624; lrenox from Lactobacillus reuteri | sorbitol DH; NAD(P)H oxidase | Padh | overcoming inhibitory effect of NADPH formation during conversion of d-sorbitol to l-sorbose in G. oxydans KCTC 1091 | Kim et al. 2019 |
| fdhSCL from G. japonicus NBRC3260; inv1417 from G. japonicus LMG 1417 |
membrane-bound fructose DH (Fdh); β-fructofuranosidase / invertase |
PGOX0264 |
production of 5-ketofructose (5-KF) by G. oxydans and G. japonicus; production of 5-KF from sucrose by invertase and Fdh with recombinant G. oxydans strains |
Hoffmann et al. 2020 |
| fdhSCL from G. japonicus NBRC3260 | membrane-bound fructose DH (Fdh) | PGOX0264 | production of 5-ketofructose from sucrose by fructose DH and invertase with G. oxydans IK003.1 | Battling et al. 2020 |
| levS1417 from G. japonicus LMG 1417 | levansucrase | PGOX0264 | high yield production of levan-type fructans by G. japonicus LMG 1417 | Hövels et al. 2020 |
| pBBR1MCS-4 | ||||
| sldAB from G. oxydans 621H | membrane-bound sorbitol DH (mSLDH) | PtufB, PsldAB | growth performance and DHA production by engineered G. oxydans 621H | Lu et al. 2012 |
| fdhSCL from G. japonicus NBRC3260 | flavoprotein-cyt. c complex fructose DH (FDH) | PadhAB | production of FDH in G. oxydans NBRC12528 strains; 5-keto-d-fructose formation | Kawai et al. 2013 |
| aroQ from G. oxydans NBRC3244 | type II 3-dehydroquinate dehydratase | Plac, ParoQ |
biotransformation of quinate to 3-dehydroshikimate by G. oxydans NBRC3244 |
Nishikura-Imamura et al. 2014 |
| kgdSLC from G. oxydans NBRC3293 | 2-ketogluconate DH | PadhAB | production of 2,5-diketo-d-gluconate by G. japonicus NBRC3271 | Kataoka et al. 2015 |
| sldBA from G. frateurii NBRC101659 | membrane-bound PQQ-dependent glycerol / polyol DH (GLDH) | Plac | sugar oxidase activities of GLDH in G. frateurii NBRC101659 strains | Yakushi et al. 2018c |
| steP from G. frateurii NBRC101659 | sugar-transporting / exporting permease | none, Plac, PGOX0284, PGOX0452 | SNP in steP affects trehalose efflux / sorbose uptake; NADPH/NADP+ ratio and thermotolerance of G. frateurii | Matsumoto et al. 2018 |
| quiA from G. oxydans NBRC3292 and NBRC3244 | membrane-bound PQQ-dependent quinate DH (QDH) | Plac, PadhAB, PquiA | improving QDH activities, tested in G. oxydans NBRC12528 and G. frateurii NBRC101659 strains | Yakushi et al. 2018b |
| sndh from Ga. liquefaciens RCTMR10 | membrane-bound sorbosone DH (SNDH) | PadhAB | expression in G. oxydans NBRC12528 and identification of the prosthetic group of SNDH | Yakushi et al. 2020 |
aIf no promoter or no native upstream region of the cloned gene(s) is mentioned in the publication, promoter Plac present on the pBBR1MSC-based plasmids was assumed to be responsible for target gene expression