Table 2.
Contents of pentacyclic triterpenoids in cultures of M. alcaliphilum grown at different salinities
| Compound | 1% NaCl (n = 3) | 3% NaCl (n = 3) | ||
|---|---|---|---|---|
| Content (µg/g dw) | SD | Content (µg/g dw) | SD | |
| diploptene XIII | 28 | 12 | 21 | 6 |
| hop-21-ene XIV | 16 | 6 | 10 | 3 |
| 3-me-diploptene XV | 6 | 3 | 5 | 2 |
| 3-me-hop-21-ene XVI | 2 | 0 | 3 | 1 |
| diplopterol XVII | 72 | 39 | 40 | 22 |
| 3-me-diplopterol XVIII | 15 | 1 | 12 | 5 |
| tetrahymanol XIX | 55 | 2 | 50 | 14 |
| 3-me-tetrahymanol XX | 2 | 1 | 4 | 2 |
| aminodiol Ia | 12 | 4 | 0 | 0 |
| aminotriol a IIIaa | 9 | 1 | 3 | 5 |
| aminotriol b IIIba | 420 | 243 | 172 | 34 |
| 3-me-aminotriol a IVaa | 71 | 28 | 8 | 6 |
| 3-me-aminotriol b IVba | 117 | 70 | 63 | 20 |
| aminotetrol Va | 93 | 49 | 40 | 8 |
| 3-me-aminotetrol VIa | 127 | 52 | 114 | 26 |
| Sum pentacyclic triterpenoids | 1045 | 413 | 544 | 88 |
| 3-me-diploptene/diploptene | 0.20 | 0.03 | 0.23 | 0.04 |
| 3-me-diplopterol/diplopterol | 0.18 | 0.04 | 0.29 | 0.04 |
| 3-me-tetrahymanol/tetrahymanol | 0.03 | 0.01 | 0.09 | 0.01 |
| 3-me-aminotriol/aminotriol b | 0.28 | 0.04 | 0.37 | 0.04 |
| 3-me-aminotetrol/aminotetrol | 1.35 | 0.49 | 2.87 | 0.13 |
| 3-methylhopanoids/hopanoids | 0.43 | 0.10 | 0.77 | 0.03 |
aBHPs were measured as products of periodic acid cleavage (aminotetrol yielded homohopanol, aminotriols yielded bishomohopanols, aminodiol yielded trishomohopanol). Bold values show statistically significant difference with respect to standard growing conditions (3% NaCl) using unpaired t test (α < 0.05). SD standard deviation, dw dry weight