Figure 6. ENPP1 expression is associated with reduced lymphocytic infiltration in human cancer.

(A) Percentage of tumor-infiltrating lymphocytes (TILs) in breast tumors stratified based on their ENPP1 expression, bars represent mean ± s.e.m., *** p < 0.001, two-tailed t-test. (B) Representative images of human breast cancers stained using anti-ENPP1 or anti-CD8 antibodies. Scale bar 100μm. (C) Tumor immune infiltration inferred using the CIBERSORT method on breast tumors found in the TCGA, box plots represent median, lower and upper quartiles, error bars represent 10^th and 90^th percentiles, n = 1079 tumors, **** p<0.0001, two-sided Mann-Whitney test. (D) Percent objective response rate (ORR) to anti-PD1/PD-L1 therapy as a function of ENPP1 expression by cancer type for tumor histologies with high levels of CGAS expression. (E) Schematic illustrating the consequence of ENPP1 activity (right) or its absence (left) on cancer metastasis and immune evasion.