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. Author manuscript; available in PMC: 2022 May 4.
Published in final edited form as: Cell Metab. 2021 Apr 21;33(5):905–922.e6. doi: 10.1016/j.cmet.2021.03.025

Figure 3. The metabolic effects of Ile restriction are independent of hepatic mTORC1 and GCN2 activity.

Figure 3.

(A) Western blot analyses of mTORC1 signaling in the liver of fasted mice after 3 weeks of feeding the indicated diets. (B) Experimental scheme. (C) Glucose tolerance in WT and L-TSC1 KO mice fed the diets for 3 weeks (n=7–8/group; for AUC, statistics for the overall effects of genotype, diet, and the interaction represent the p value from a two-way ANOVA, *p<0.05, from a Sidak’s post-test examining the effect of parameters identified as significant in the two-way ANOVA). (D) Heatmap depiction of the metabolic effects of the indicated diets in WT and L-TSC1 KO mice. (E) Western blot analyses of GCN2 signaling in the liver of fasted mice after 3 weeks. (F) Experimental scheme. (G) Glucose tolerance in WT and L-GCN2 KO mice fed the indicated diets for 3 weeks (n=6–7/group; for AUC, statistics for the overall effects of genotype, diet, and the interaction represent the p value from a two-way ANOVA, *p<0.05, from a Sidak’s post-test examining the effect of parameters identified as significant in the 2-way ANOVA). (H) Heatmap of the metabolic effects of each diet. Data represented as mean ± SEM.