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. Author manuscript; available in PMC: 2022 May 4.
Published in final edited form as: Lab Chip. 2021 May 4;21(9):1706–1723. doi: 10.1039/d1lc00119a

Figure 9.

Figure 9.

Validation of iFCS with canine and human cancer patients’ blood sample. (A) Cytopathological staining of isolated CTC from a canine patient with stage II osteosarcoma. CTCs were indicated by dotted green circles; WBCs were indicated by dotted red circles. Scale bar: 10 μm. (B) Bright-field and immunofluorescence images of 3 selected CTCs and 1 WBC separated from 2 human patients with stage IV breast cancer. Four channels were used in immunofluorescence staining, including the CTC marker cytokeratin (CK, green), leukocyte marker CD45 (red), and nucleus marker DAPI (blue). Cells were identified as CTC if the staining pattern is CK+/CD45−, WBCs were identified as CK−/CD45+. Scale bar: 10 μm.