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. Author manuscript; available in PMC: 2021 Oct 27.
Published in final edited form as: Nat Immunol. 2021 Apr 27;22(5):639–653. doi: 10.1038/s41590-021-00922-4

Extended Data Fig. 6. Single cell analysis identifies unique myeloid populations within healthy human WAT.

Extended Data Fig. 6

(a) Representative sorting strategies for CD11b+CD14+ macrophage and HLA-DR+CD11c+ dendritic cell populations indicated in Figure 4. (b) Heatmap shows the top 4 differentially expressed cluster marker genes for each indicated myeloid cell cluster. Cluster identities are shown above the heatmap. Color saturation indicates the strength of expression. (c,d) Violin plots showing RNA expression levels of cluster markers for (c) myeloid (d) and monocyte populations. (e) Classical, Intermediate, and Nonclassical Monocyte gene module score analysis for the indicated monocyte populations based on comparison of signature genes for each cell type from previously defined datasets to DEGs within each cluster. (f) Neutrophil gene module score analysis for the indicated myeloid populations based on comparison of signature genes for each cell type from previously defined datasets to DEGs within each cluster. (g) Bar plots showing the proportion of myeloid cells derived from 7 lean (blue) and 5 obese (red) patients.