Uniaxially aligned PLGA nanofibers with graded mineralization for spatial control of osteogenesis. A) SEM images of calcium phosphate coatings on a PLGA nanofiber mat at i) 1 cm, ii) 2 cm, iii) 3 cm, and iv) 5 cm away from the unmineralized end of the scaffold. B) EDX quantification of the gradient in mineral content (n = 3). C) ASCs seeded on the aligned nanofibers with a spatial mineral gradient after culture for 28 days by i–iii) Runx2 and iv–vi) OCN staining, respectively. A–C) Adapted under the terms of the ACS AuthorChoice license.[54] Copyright 2014, American Chemical Society.