Figure 7.

Metabolic task analysis of WT versus knockout mouse kidney transcriptomics indicates OAT1 (but not OAT3) dependence of five tryptophan-related metabolic tasks.A, the metabolic task analysis uses transcriptomic data to predict cellular functions. B, the 13 tasks displayed have a coefficient of variation (standard deviation/mean) above 15% and a mean metabolic task score greater than 0.5 across both knockouts. Included in these are five tryptophan-related tasks (synthesis of anthranilate, synthesis of kynurenate, synthesis of kynurenine, synthesis of N-formylanthranilate, and synthesis of quinolinate) that have increased metabolic task scores in the Oat1 KO (n = 3) compared with wild type (n = 3) and decreased metabolic task scores in the Oat3 KO (n = 3) compared with wild type (n = 3). A score of 0 suggests low metabolic activity and a score of 7 suggests high metabolic activity.