Table 1.

Morphological and contractile properties of soleus (Sol) muscles at 7 days after CTX injection

Group		Sol
	Mouse mass (g) ± SEM	Muscle mass (mg) ± SEM	L0 (mm) ± SEM	Pt (g) ± SEM	½ RT (ms) ± SEM	TPT (ms) ± SEM
Sham	28.6 ± 2.0	8.1 ± 0.3	12.3 ± 0.3	3.7 ± 0.4	42.6 ± 3.4	36.6 ± 2.2
CTX-PBS	25.7 ± 0.6	6.2 ± 0.3∗∗	11.3 ± 0.4	2.3 ± 0.2∗∗	45.0 ± 2.8	35.6 ± 1.6
CTX-hFL-OPG-Fc	25.7 ± 0.6	6.2 ± 0.3∗∗	11.6 ± 0.2	2.8 ± 0.2	41.6 ± 2.8	34.8 ± 2.4

The Sol muscles were incubated ex vivo in a controlled physiological environment (Krebs-Ringer solution supplemented with 95% O₂ and 5% CO₂, pH 7.4) at 25°C. L₀ values were determined, and the twitch tension (Pt), half-relaxation time (½ RT), and time to peak tension (TPT) were recorded. The muscle masses of Sol muscles from CTX-treated mice were significantly lower than those of Sol muscles from sham mice, while the Pt was significantly lower only in the PBS-treated group. No other morphological and contractile parameters were significantly different. Data are expressed as means ± SEM. ∗∗p < 0.01, indicate significantly different from sham mice using a one-way analysis of variance ANOVA with a Dunnett’s test (one-way ANOVA) for multiple comparisons (n = 7–9).