Figure 2.

Hypoxia causes a metabolic shift in CD34⁺ hematopoietic stem and progenitor cells. (A) Principal component analysis of samples on the basis of fold change in metabolites in spent medium relative to fresh medium from CD34⁺ cell cultures incubated in hypoxia or normoxia. (B) Fold change in glucose and lactate in spent medium relative to fresh medium from CD34⁺ cell cultures incubated in normoxia or hypoxia. (C) Fold change in amino acids in spent medium relative to fresh medium from CD34⁺ cell cultures incubated in normoxia or hypoxia. Spent medium was collected on day 4 of culture. Statistical analysis was performed in MetaboAnalyst, and p values ≤ 0.05 were considered significant. *p < 0.05, **p < 0.005, ***p < 0.0005.