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. Author manuscript; available in PMC: 2021 May 7.
Published in final edited form as: Exp Hematol. 2021 Mar 3;97:32–46.e35. doi: 10.1016/j.exphem.2021.02.012

Figure 3.

Figure 3.

Hypoxia promotes the development of megakaryoerythroid progenitors. (A) Schematic of myeloerythroid differentiation from hematopoietic stem cells. The surface markers used for immunophenotyping of the cells interrogated in this study are indicated. (B) Percentage of multipotent progenitors (MPP), common myeloid progenitors (CMP), megakaryoerythroid progenitors (MEP), and granulocyte−monocyte progenitors (GMP) in cultures incubated in hypoxia and normoxia. For MPPs, the percentage of positive cells in the Lin−/Live population was determined, and for CMPs, GMPs, and MEPs, the percentage of positive cells in the MPP population was determined on days 1, 7, 14, and 21. Data are represented as the mean with standard error (n = 4). Statistical analysis was performed using the Mann-Whitney test, and p values ≤ 0.05 were considered significant. (C) tSNE analysis was performed on flow cytometry standard files. tSNE plots for day 21 analysis revealing CD34+ and CD38+ cells in normoxia and hypoxia are represented in the Lin−/Live population. (D) tSNE analysis plots revealing the distribution of CMPs, GMPs, and MEPs in the CD34+/CD38+ population in normoxia or hypoxia on day 21 are represented. (E) Histograms for CD45Ra and CD123 expression indicating relative distribution of CMPs (CD45Ra−/CD123lo), GMPs (CD45Ra+/CD123lo), and MEPs (CD45Ra−/CD123−) in normoxia and hypoxia. *p < 0.05, **p < 0.005, ***p < 0.0005.