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. 2021 May 6;253(6):117. doi: 10.1007/s00425-021-03634-z

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© The Author(s) 2021

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Fig. 2 — Analysis of Zn tolerance and accumulation in wild type (WT) and ScZRC1-expressing A. thaliana plants. Seeds were germinated on standard MS solid medium and grown in a growth chamber for 2 weeks. Plantlets were transferred in hydroponics for two further weeks, in standard Hoagland’s solution (non-treated control) and in Hoagland’s solution amended with 20 µM ZnSO₄ (Zn treated). To estimate Zn tolerance, root length (a) and total chlorophyll content (b) were measured. Zn accumulation was determined in shoots (c). Different letters above the histograms indicate statistical significance, evaluated by two-way ANOVA followed by a post hoc Bonferroni test (P < 0.05, n = 30 for root length and n = 4 pools for chlorophyll content and Zn accumulation)