Figure 6.

Schematic representation of the effect of different solution conditions (pure buffer and different salts (60 mM NaCl, KCl)) and pressure on the conformation of the 22AG quadruplex in the absence and presence of the ligand ThT. Owing to the complexity of the system, no sketch of the Ficoll data are shown here. For sake of clarity, the possibility of multiple binding modes (as mainly occurring at high pressures) is not taken into account in this schematic, only one representative binding site is shown here. The (apparent) volume changes for the overall reaction (ΔV_r) and tentative values of the ThT binding step (ΔV_b) and the conformational transitions (ΔV_tr) of the GQ are also indicated.