Table 4.

Reaction volumes (ΔV_r) for the complex formation between 22AG and ThT at the temperature of 25 °C. The ΔV_r values were obtained from the slope of lnK_b vs. p, which yields—ΔV_r/(RT).

Solvent	ΔVr/mL mol−1
30 mM Tris, pH 7.4	− 11.2 ± 0.7
+ 60 mM NaCl	− 19.1 ± 0.6
+ 60 mM KCl	− 4.2 ± 0.8
+ 25 wt% Ficoll	4.0 ± 2.1/− 7.2 ± 0.3a

^aThe first value and the second value refer to the volume change in the 1–1000 bar and 1000–2000 bar pressure range, respectively.