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. 2021 May 6;11:9644. doi: 10.1038/s41598-021-88959-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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TGFBI modulates vessel infiltration into Matrigel containing Ad-CM. Matrigel impregnated with either normal medium or Ad-CM was injected in WT and KO mice. Normal medium: DMEM-high glucose, 10% FBS, and 1% antibiotics. (a) Schematic of the Matrigel plug assay in WT and TGFBI KO mice treated with Ad-CM. (b) Representative H&E staining section of Matrigel from WT and KO mice (n = 3/group). Scale bars represent 100 μm. (c) The infiltrated vessels were counted in three Matrigel H&E-stained sections and expressed as the mean number. Significant differences were evaluated between two groups of experiments in the same condition (WT-NON versus KO-NON or WT-CM versus KO-CM). (d) Relative hemoglobin content of Matrigel plugs. The insert image represents Matrigel for each group. Data are presented as the mean ± s.e.m. **p < 0.01 versus control.