Table 3.
Gene expression analysis in HLEC, dLEC and HTR-8/SVneo. mRNA expression of enzymes involved in progesterone metabolism in HLEC, dLEC and HTR-8/SV neo. Expression levels are shown as ct values per 12.5 ng cDNA.
Yellow: significant expression (ct < 35).
Blue: no or very low expression (ct ≥ 35).
SRD5A1 (5α-reductase isoform 1), SRD5A2 (5α-reductase isoform 2), SRD5A3 (5α-reductase isoform 3), AKR1D1 (5β-reductase), AKR1C1 (20α-HSD), AKR1C2 (3α-HSD3), AKR1C3 (HSD17B5), AKR1C4 (3α-HSD1), HSD3B1, HSD3B2. Appropriate positive and negative controls were run.