Table 5.

Gene expression analysis in HLEC, dLEC and HTR-8/SVneo. mRNA expression of steroidogenic enzymes involved in de-novo steroid hormone production in HLEC, dLEC and HTR-8/SV neo. Expression levels are shown as ct values per 12.5 ng cDNA. Yellow: significant expression (ct < 35). Blue: no or very low expression (ct ≥ 35). CYP17A1 (steroid-17α-hydroxylase), CYP21A2 (steroid-21-hydroxylase), CYP11B1 (steroid-11β-hydroxylase), CYP11B2 (aldosterone synthase), HSD11B1 (11β-hydroxysteroid-dehydrogenase 1), HSD11B2 (11β-hydroxysteroid-dehydrogenase 2), HSD17B2 (17β-hydroxysteroid dehydrogenase 2). Appropriate positive and negative controls were run.