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. 2021 May 6;12(5):453. doi: 10.1038/s41419-021-03736-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A Western blot analysis of SIRT1, TM4SF1, and p21 in DOX-treated shB7-H3 CRC cells cotreated with TM4SF1 overexpression vectors and SIRT1 siRNA. β-Actin served as a loading control. B, C SA-β-Gal activity of DOX-treated shB7-H3 CRC cells with TM4SF1 overexpression vectors and SIRT1 siRNA cotreatment was examined. Scale bar, 100 μm. One representative image from three reproducible experiments is shown. The percentages of SA-β-gal-positive cells are shown in the bar graph. D, E SAHF activity of DOX-treated shB7-H3 CRC cells with TM4SF1 overexpression vectors and SIRT1 siRNA cotreatment was examined. Scale bar, 50 μm. One representative image from three replicate experiments. F The cell viability of control cells or DOX-treated shB7-H3 CRC cells cotreated with TM4SF1 overexpression vectors and SIRT1 siRNA after 24, 36, 48, and 96 h was examined by CCK-8 assays. G The colony formation of control cells or DOX-treated shB7-H3 CRC cells with TM4SF1 overexpression vectors and SIRT1 siRNA cotreatment was examined. One representative image from three reproducible experiments is shown. The number of colonies is shown in the bar graph. H Cell cycle analysis by PI staining in control cells or DOX-treated shB7-H3 CRC cells with TM4SF1 overexpression vectors and SIRT1 siRNA cotreatment was examined through flow cytometry. The data represent the means ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001.